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16s rrna gene amplification  (ATCC)


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    Structured Review

    ATCC 16s rrna gene amplification
    16s Rrna Gene Amplification, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/16s+rrna+gene/16S/pm42268876-62-1-20
    Average 99 stars, based on 6294 article reviews
    16s rrna gene amplification - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Draft genome sequence of Listeria innocua AZLIn05 isolated from beef seekh kebab in Bangladesh.
    Article Snippet: .. Comparative analysis of the 16S rRNA gene using BLASTn showed 100% sequence identity and 97% query coverage with L. innocua strain ATCC 33090. ..

    Article Title: Genome-Informed Characterization of Lactiplantibacillus plantarum ND88 with Inhibitory Activity against Representative Oral Pathogens.
    Article Snippet: .. A BLAST analysis of the 16S rRNA gene obtained from the whole-genome sequence revealed 100% identity (1489/1489 bp) with the 16S rRNA gene of L. plantarum ATCC 14917, confirming the taxonomic designation of the strain as ND88. into three contigs with an N50 value of 3,197,760 bp and an average sequencing coverage of 100 × using SMRT Link v10.1.0 followed by Unicycler v0.4.9 for assembly refinement. ..

    Amplification:

    Article Title: Detection and Identification of Paragyliauchen sp. (Trematoda) and Bacteria in Diseased Centropyge bicolor from the European Marine Aquarium Trade.
    Article Snippet: .. Therefore, a fragment of the 16S rRNA gene was amplified using conventional PCR, and the resulting amplicon was subsequently sequenced, revealing 99.55% identity of the isolate to the reference strain B. diminuta ATCC 11568 (Table 4). ..

    Article Title: Genetic characterization and mutagenesis of fat-degrading enzymes from Bacillus cereus for enhanced lipid degradation and esterification.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Article Title: Systems-level restoration of vaginal and gut microbiota by lactobacillus helveticus 20 838 alleviates Gardnerella vaginalis-induced dysbiosis.
    Article Snippet: .. The hypervariable regions of the bacterial 16S rRNA gene were amplified using a forward primer (5′- TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG3′) and a reverse primer (5′- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTA ATCC-3′). .. PCR amplification was performed using 2× KAPA HiFi HotStart ReadyMix (Roche, Basel, Basel-Stadt, Switzerland), with an initial denaturation at 95 °C for 3 minutes, followed by 25 cycles of 95 °C for 30 seconds, 55 °C for 30 seconds, and 72 °C for 30 seconds, and a final extension at 72 °C for 5 minutes.

    Polymerase Chain Reaction:

    Article Title: Detection and Identification of Paragyliauchen sp. (Trematoda) and Bacteria in Diseased Centropyge bicolor from the European Marine Aquarium Trade.
    Article Snippet: .. Therefore, a fragment of the 16S rRNA gene was amplified using conventional PCR, and the resulting amplicon was subsequently sequenced, revealing 99.55% identity of the isolate to the reference strain B. diminuta ATCC 11568 (Table 4). ..

    Article Title: Gut Commensals Regulate the Intestinal Kynurenine Pathway
    Article Snippet: .. A PCR product of the 16S rRNA gene from Enterococcus faecium ATCC 700221 strain was used for obtaining a standard curve by amplifying its 16S rRNA gene and purifying the product. ..

    Positive Control:

    Article Title: Machine Learning Analysis of Type VII Secretion System Expression and Its Relationships With Virulence Traits and Antibiotic Resistance in Staphylococcus aureus
    Article Snippet: .. The 16S rRNA gene of S. aureus was used as the positive control, and E. coli ATCC 25922 was used as the negative control. ..

    Negative Control:

    Article Title: Machine Learning Analysis of Type VII Secretion System Expression and Its Relationships With Virulence Traits and Antibiotic Resistance in Staphylococcus aureus
    Article Snippet: .. The 16S rRNA gene of S. aureus was used as the positive control, and E. coli ATCC 25922 was used as the negative control. ..

    Electrophoresis:

    Article Title: Genetic characterization and mutagenesis of fat-degrading enzymes from Bacillus cereus for enhanced lipid degradation and esterification.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    Predicted functional pathways based on KEGG annotations associated with H. pylori infection and eradication status. (A) Relative representation of predicted pathways between H. pylori –negative and H. pylori –positive samples **, P < 0.01; ***, P < 0.001 for two-group comparison. (B) Relative changes in predicted functional pathways between baseline and follow-up samples across eradicated, recurrent GC after eradication, and non-eradicated groups. Functional profiles were predicted from <t>16S</t> <t>rRNA</t> gene sequencing data using KEGG-based pathway inference. *, P < 0.05; ***, P < 0.001 for comparison between baseline and follow-up in each group. KEGG, Kyoto Encyclopedia of Genes and Genomes.
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    Azenta 16s rrna gene sequencing
    Heatmap showing the relative abundance of identified PGPR-associated metabolic pathways across five samples (Root_13, Root_14, Humus_4 (surface), Humus_5 (middle depth), and Humus_6 (deep), inferred from <t>16S</t> <t>rRNA</t> gene sequencing data using PICRUSt2. Pathways are hierarchically clustered (dendrograms) based on similarity in predicted functional profiles. The color gradient represents normalized relative abundance values, ranging from −1 (blue, lower abundance) to 1.5 (red, higher abundance), with intermediate levels shown in white/yellow.
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    Image Search Results


    Predicted functional pathways based on KEGG annotations associated with H. pylori infection and eradication status. (A) Relative representation of predicted pathways between H. pylori –negative and H. pylori –positive samples **, P < 0.01; ***, P < 0.001 for two-group comparison. (B) Relative changes in predicted functional pathways between baseline and follow-up samples across eradicated, recurrent GC after eradication, and non-eradicated groups. Functional profiles were predicted from 16S rRNA gene sequencing data using KEGG-based pathway inference. *, P < 0.05; ***, P < 0.001 for comparison between baseline and follow-up in each group. KEGG, Kyoto Encyclopedia of Genes and Genomes.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Longitudinal remodeling of gastric microbiota following Helicobacter pylori eradication reveals an eradication-associated microbial signature in gastric cancer

    doi: 10.3389/fcimb.2026.1848437

    Figure Lengend Snippet: Predicted functional pathways based on KEGG annotations associated with H. pylori infection and eradication status. (A) Relative representation of predicted pathways between H. pylori –negative and H. pylori –positive samples **, P < 0.01; ***, P < 0.001 for two-group comparison. (B) Relative changes in predicted functional pathways between baseline and follow-up samples across eradicated, recurrent GC after eradication, and non-eradicated groups. Functional profiles were predicted from 16S rRNA gene sequencing data using KEGG-based pathway inference. *, P < 0.05; ***, P < 0.001 for comparison between baseline and follow-up in each group. KEGG, Kyoto Encyclopedia of Genes and Genomes.

    Article Snippet: The raw 16S rRNA gene sequencing data generated in this study have been deposited in the National Center for Biotechnology Information Sequence Read Archive under the BioProject accession number PRJNA1447187 and are publicly available at http://www.ncbi.nlm.nih.gov/bioproject/1447187 .

    Techniques: Functional Assay, Infection, Comparison, Sequencing

    Heatmap showing the relative abundance of identified PGPR-associated metabolic pathways across five samples (Root_13, Root_14, Humus_4 (surface), Humus_5 (middle depth), and Humus_6 (deep), inferred from 16S rRNA gene sequencing data using PICRUSt2. Pathways are hierarchically clustered (dendrograms) based on similarity in predicted functional profiles. The color gradient represents normalized relative abundance values, ranging from −1 (blue, lower abundance) to 1.5 (red, higher abundance), with intermediate levels shown in white/yellow.

    Journal: bioRxiv

    Article Title: Taxonomic Composition and Predicted Functional Potential of a Commercial Microbiome-Based Fertilizer Additive and Agricultural Soils in Eastern Paraguay

    doi: 10.64898/2026.06.03.729874

    Figure Lengend Snippet: Heatmap showing the relative abundance of identified PGPR-associated metabolic pathways across five samples (Root_13, Root_14, Humus_4 (surface), Humus_5 (middle depth), and Humus_6 (deep), inferred from 16S rRNA gene sequencing data using PICRUSt2. Pathways are hierarchically clustered (dendrograms) based on similarity in predicted functional profiles. The color gradient represents normalized relative abundance values, ranging from −1 (blue, lower abundance) to 1.5 (red, higher abundance), with intermediate levels shown in white/yellow.

    Article Snippet: 16S rRNA gene sequencing was performed by Azenta Life Sciences (South Plainfield, NJ, USA) using their 16S-EZ service.

    Techniques: Sequencing, Functional Assay